Examination of samples with light microscopy reveals critical information using a variety of magnifications, and illumination configurations.

Optical Microscopy Utilizing Polarized Light

Polarized light microscopy (PLM) is a powerful and flexible technique for the examination of small particles such as pigment particles, mineral grains, fibers, environmental particulate, and biological materials. Light microscopy has been used for many decades to characterize particles down to the micrometer size scale.

> PLM Applications

> For Pigment Analysis - PLM provides refractive index, particle size, size distribution, color, shape, pleochroism, optical properties, and morphology data which are critical for the identification of the type of pigment and its source.

> For Fiber Analysis—PLM reveals surface characteristics, fiber diameter and optical properties such as refractive index and birefringence that can discriminate fibers that are identical chemically, but have morphological differences.

 

Images of quartz grains in transmitted light (top), with crossed polars (middle) and with crossed polars and 1st order red plate (bottom)

Polarized Light Microscopy

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Center for Art Materials Analysis, Inc.

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polarized light microscopy (PLM) can be used for pigment analysis, art authenticaion, and general materials analysisquartz crystals examined with polarized light microscopy shows crystal color, shape, morphology when examinedquartz crystals examined with polarized light microscopy shows birefringence when examined with crossed polarsquartz crystals examined with polarized light microscopy shows birefringence when examined with crossed polars, additional first order red plate shows optic sign

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